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To this end, we optimized the viability stain FUN 1 and operating parameters of the microarray scanner such that the fluorescence intensity correlated linearly with cell number over the range of interest (Figure 4A).
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Finally, fluorescence images were captured using a microarray scanner (Axon).
Slides were scanned on an Agilent G2505C DNA microarray scanner.
The BeadChips were analyzed using the Illumina iScan N240 microarray scanner (Illumina, CA, USA).
The samples were finally transferred to a microarray scanner for further LSC analysis.
The chip images were obtained by LuxScanTM 10 K Microarray Scanner (CapitalBio, Beijing, China) using Cy3 settings.
After washing, the slide image files were generated by a DNA microarray scanner (G2505B; Agilent Technologies).
The arrays were scanned using an Agilent Single-Color DNA Microarray Scanner (Agilent Technologies).
Array scanning and feature extraction was performed using the Agilent G2565CA microarray scanner as outlined in the Agilent protocol for Two-colour Microarray-Based Gene Expression Analysis, Version 6.5, May 2010.
Image file of fluorescent signal on the slide was produced with a DNA microarray scanner (Agilent Technologies).
Microarrays were scanned using an Agilent DNA microarray scanner G2505B.
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