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Arrays were washed, dried and scanned on an Agilent G2565BA microarray scanner described by [ 9].
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Cy5-sample cRNA and Cy3-common reference cRNA were generated and hybridised to a Whole Human Genome oligo DNA microarray kit (Agilent Technologies), which was scanned using an Agilent DNA microarray scanner (Agilent), as described previously (Takeuchi et al, 2006).
We performed: indirect labelling of the tumor total RNA-derived cDNA (Cy5) and reference RNA-derived cDNA (Cy3); microarray hybridization and washing; and scanning with GenePix 4000 microarray scanner and GenePix software as previously described [67].
Following hybridization and washing, microarrays were imaged using a DNA microarray scanner (agilent technologies) and array analysis and quality control were performed as described in [8].
Microarray chips were scanned using a DNA Microarray Scanner (Agilent Technologies).
Afterwards, microarrays were washed using Agilent Gene Expression Wash Buffer Kit and scanned with the Agilent DNA microarray scanner G2505B.
The microarrays were dried by centrifugation (1,500 rpm, 5 min) and scanned immediately in an Agilent microarray scanner.
Fluorescence detection of cytokines was performed on a GenePix 4000B Microarray scanner.
Following washing steps the slides were scanned using the Axon GenePix 4000B microarray scanner.
After washing with Gene Expression Wash Buffers Pack (Agilent Technologies), the hybridization images were analysed using an Agilent Microarray Scanner (G2565CA) (Agilent Technologies).
GeneChips were washed using the Gene Expression Wash Buffers Pack and scanned using an Agilent DNA Microarray Scanner (G2565CA).
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