In total, therefore, the composite dataset consisted of 550 microarray samples, including representatives from 10 cell types in addition to normal human skin.
A total of 150 NSCLC tissue microarray samples, including 82 cases of lung adenocarcinoma and 68 cases of lung squamous cell carcinomas (SCCs), were collected from Shanghai Outdo Biotech Co. Ltd. between Dec. 2004 and Jan . 2008
Samples corresponded to those compared using microarrays plus some extra samples included for increasing the number of biological replications per tissue.
For one, the scarce number of samples included in the microarray analysis may limit the validity of the array results.
Microdissected tissues and 4 paired normal-tumoral samples included in the microarray analysis were used for Q-PCR analyses.
The CIS samples included in the microarray data analysis originated from tissue next to different overt tumours (Almstrup et al, 2004).
Automated electrophoresis qualities confirmed RQI (RNA Quality Index) values > 8.0 for all samples included in our microarray analysis (Additional file 1: Figure S1).
In order to increase the power of the analysis, 9 control and 20 RSV-positive samples included in the microarray were also included in the qPCR.> -wrap-foot> NoTherehere are no significant differences in age, weight or gender between control and RSV-positive groups.
To maximize the uniformity of the microarray data, all the samples, including the ones analyzed by DNA microarray previously, were hybridized to the same platform (the NIA Mouse 44K Microarray v3.0 manufactured by Agilent Technologies #015 015).
Each microarray contains 159 thyroid tissue samples, including 81 thyroid carcinomas, 38 thyroid adenomas, and 40 normal thyroid tissues.
