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According to the microarray results, this transgene caused a significant up-regulation of numerous (a total of 1354) transcripts, with induction levels ranging up to 3000-fold level.
In accordance with the microarray results, this gene had a 20% increase in expression in the duodenum of the susceptible animals compared to the resistant.
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This database provides experimental microarray results for this organism for four stress conditions: heat shock [28], pH [29], UV exposure [30] and tryptophan metabolism [31].
Our microarray results extend this observation to the larvae and adult hearts as well as to another cell culture line, the PAC2 cell line.
Conflicting results have been reported with regard to the reliability of microarray results using this method.
All microarray results from this study were deposited in NCBI's Gene Expression Omnibus (GEO) database [ 22].
Our microarray results reflect this to some extent, with considerably more genes downregulated in the liver than upregulated.
Microarray results with this "Reference" data set should reflect transcript levels in the average differentiated embryonic cell.
The reference gene was selected because microarray results for this gene showed stable values over all tissues.
However, the results for IL6 cluster together, illustrating the consistency of the disparity between qRT-PCR and microarray results for this transcript.
Our microarray results confirm this, as Foxh1 levels are not changed in Foxa2 mutants; a result that was further validated using Q-PCR (Additional File 1).
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