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Yet, over 77% of unknown contigs and 65% of unknown singletons are transcribed as determined by microarray analysis (see section on microarray results), suggesting that they may represent protein-coding genes as well as non-coding trasncripts such as microRNAs and long non-coding RNAs.
They state, "These data were consistent with the microarray results".
This was in line with the positive microarray results.
Microarray results were validated using real-time RT-PCR.
(A) Validation of the microarray results in both MKN-45 and SGC-7901 cells using qRT-PCR.
Validation of microarray results by semi-quantitative RT-PCR revealed temporal variation in gene expression profiles.
Fig. 4 Validation of microarray results by semi-quantitative RT-PCR.
Microarray results suggested that OsIRO3 expression may be positively regulated by IDEF1 (Table 1).
Details of genes shortlisted, on the basis of microarray results, for semi-quantitative RT-PCR.
For confirmation of the microarray results, qRT-PCR was conducted, and the results are demonstrated in Fig. 3.
Selection of the reference genes was based on the microarray results using an algorithm described in Popovici et al. (2009).
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