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In the microarray results the probe for oligophrenin-1, a gene known for its involvement in mental retardation, showed a decreased hybridization signal.
In accordance with the microarray results the expression of VTG, ZP3 and nm23 were significantly induced in fish exposed to the high concentration.
To confirm the accuracy of the microarray results, the transcript abundance of 13 selected genes was carried out.
For validation of the microarray results, the same total RNA as that used in the microarray experiments was analyzed.
To validate the microarray results, the MCF-7 cells were treated in a separate experiment using the identical experiment conditions and method as described above.
Although the magnitudes of fold changes in laccase gene expression were much higher than the microarray results, the real-time RT-PCR data supported those of the microarray analyses.
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The microarray results further support this conclusion.
Therefore, the analysis of selected miRNAs by qPCR confirmed the microarray results, indicating the quality of the miRNA microarray.
JW analyzed the microarray results, drafted the manuscript, and coordinated the study.
Generally, the RT-PCR results confirmed the microarray results, but the fold change was usually higher in the RT-PCR.
These observations confirmed the reliability and comparability of the microarray results in the two grapevine species.
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