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Microarray results showed that upregulated genes were associated with an activated immune response.
The microarray results showed that many genes involved in cell survival and proliferation were regulated by bFGF.
Our microarray results showed significant upregulation of master cardiac transcriptional regulators, as well as cardiac-specific structural and functional genes (Figure 2a, also see Supplemental Results S1).
The microarray results showed that transcription of cluster 6 genes was strongly repressed by HUαβ at all three growth phases analyzed (Fig. 1).
Microarray results showed 7 genes that were significantly changed ≥3-fold in at least one of the two comparisons (Table 6).
The microarray results showed that there were many fewer significantly altered genes following IH (12 up-regulated and 4 down-regulated genes) (Table S1) as compared to CH (94 up-regulated and 70 down-regulated genes) (Table S2).
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The microarray results show that our modified technologies are useful to enhance sensitivity and specificity of arrays, identify expression patterns within different cells, and discover differential expression of sRNAs during the differentiation process of bone marrow stem cells.
Expression data from our microarray results show high levels of miR-23a throughout the GP to OL stages.
Our RT-qPCR measurements in the same 20 samples confirmed the microarray results, showing that this microRNA is indeed down-regulated (FC = −9.4, P = 1.6 7), and a close-to-perfect separation is shown with an AUC of 0.99 (Figure 7).
Simulations and real microarray results show that false positive rate of SWang is lower than currently popular methods (T-test, F-test, SAM, and Fold-change) with much higher statistical power.
Altered expression of three other transcripts PCDH8, CTGF and EphB4 were confirmed by RT-PCR analysis (Fig. 3) in agreement with the microarray results shown in Figure 2. Confirmation of protein levels for three of the four genes selected was done based on availability of commercial antibodies.
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