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Therefore, the ISH analysis validates the microarray results reported above.
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As a result, the data for a larger number of animals and microarray results are reported here.
Results presented herein, similar to microarray results recently reported for the lacrimal gland [ 9], indicate that HPCluster analysis of the microarray data identifies multiple sets of genes whose associated pathways correlate with concepts currently hypothesized to explain the early pathophysiological processes and subsequent autoimmunity.
However, the dominant pattern observed in the comparison of microarray and qRT-PCR results reported here closely follows the line of equality, which was not observed in the large scale study [ 30].
This is consistent with in situ hybridization results reported previously [ 52] and in line with the results of our microarray analysis.
The microarray results have been reported according to the MIAME guidelines and deposited in the public repository Array-Express at http://www.ebi.ac.uk/arrayexpress.ac.uk/arrayexpress
Taken together, these data validate the microarray results, solidifying the findings reported above.
Counts of duplicated genes from this RNA-seq study were placed into the 12 expression levels categories and compared to the previously reported microarray results of petal transcriptomes (Additional file 4: Figure S4, [ 18]).
Fortunately, Turpev and colleagues have recently reported selected microarray results from NO exposure of undifferentiated U937 and Mono Mac 6 cells [ 57].
Data reported on microarray results utilized in-house Perl scripts with t-test and adjusted with B-H FDR method to examine differentially expressed genes between two groups (P < 0.05, Fold Change (FC) > 1.5).
A subset of genes was validated by qRT-PCR and results were correlated well with microarray data indicating that microarray results provided an accurate report of transcript level.
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