To verify the microarray results, quantitative real-time RT-PCR (q-PCR) was performed on RNA prepared from an independently derived set of aortic EC. 35 genes with greater than four-fold differential expression that represented the major biological processes affected were selected for verification.
To verify the microarray results, quantitative PCR assays were carried out for the 12 selected genes.
For validation of the microarray results, quantitative real time PCR (Q-RT-PCR) analyses were performed using SYBR Green.
To validate the microarray results, quantitative real time PCR (qRT-PCR) assays were performed on the same RNA samples used for the microarrays.
To validate microarray results, quantitative real-time PCR was conducted using gene-specific primers (Additional file 18) as previously described in [ 51].
To confirm the microarray results, quantitative RT-PCR was used to examine the RNA levels of eight of the genes identified as having altered regulation by MTAP.
To confirm the microarray results, quantitative Real-Time-PCR (qPCR) was performed on a subset of the genes from Tv and Ta.
In order to validate the microarray results, quantitative real-time PCR (qRT-PCR) analysis was performed on RNA extracted from the same hippocampal samples employed for microarray experiments.
To confirm our microarray results, quantitative real-time PCR (qPCR) was performed after treating INS-1E cells with 10 μM MPA for 12, 24, or 36 h.
To confirm these microarray results, quantitative Real-Time-PCR (qRT-PCR) was performed on a subset of the genes belonging to different clusters.
In accordance with the microarray results, quantitative RT-PCR experiments demonstrated higher expression of Notch2, Numb and myogenin in mdx than in control mice at all ages.
