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Assuming that microarray results provide the gold standard, we calculated false discovery rate to be 0.174% on average and false negative rate to be 0.209%.
Our gene expression microarray results provide further evidence for the role of these genes in mediating host susceptibility to S. aureus infection.
Our EST resources and microarray results provide an important starting point to begin exploring putative candidate genes that may be associated with sex-specific (such as doublesex, transformer-2 or members of the yellow gene family) or nutrition-dependent (e.g. foxo) expression of horns.
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A subset of genes was validated by qRT-PCR and results were correlated well with microarray data indicating that microarray results provided an accurate report of transcript level.
The microarray results provided novel information about genes associated with ethylene metabolism in the Spcdc25 plants.
Validation of the microarray results provided many examples of genes that could be evaluated as potential biomarkers to predict response to treatment with SINE compounds.
In conclusion, the factorial analysis of the microarray results provides important information on how their expression is influenced by important environmental factors.
In addition, our microarrays results provide preliminary evidence regarding the possible role of the genes and pathways studied in this trial.
The microarray results also provide rationale for examining additional gene products that have not yet been assigned a function, but that are shown to vary between infected and uninfected cells.
Complete microarray results are provided as an additional file 1.
Complete microarray results are provided in Additional file 1 and the data were submitted to NCBI GEO Omnibus (GSE18120).
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