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Microarray results, performed on HA-EC and HOC-EC, confirmed the endothelial origin of the isolated cells (Table 2).
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To confirm our microarray results, we performed quantitative real-time PCR analysis (Figure 6).
To validate the microarray results, we performed quantitative real-time PCR (qRT-PCR) of all the previously selected genes.
Quantitative PCR reactions to confirm microarray results were performed using the ABI 7900HT real-time PCR machine (Applied Biosystems), with each reaction containing 4 ng of reverse transcribed RNA in a 20 µl reaction.
Statistical analysis of the microarray results was performed with the use of GeneSpring 11GX application (Agilent Technologies).
Validation of microarray results was performed by quantitative real-time polymerase chain reaction (qRT-PCR analysis).
To validate the microarray results we performed quantitative Real Time-PCR (qRT-PCR).
The statistical analysis of microarray results was performed using the functions available within Acuity®.
Statistical analysis of the microarray results was performed with a GeneSpring 11 GX application (Agilent Technologies).
In order to validate the microarray results we performed real-time RT PCR.
Validation of microarray results was performed using sodium bisulfite pyrosequencing on genomic DNA from sorted nuclei.
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