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RT-qPCR was performed to verify the microarray results on the discovery sample set (n = 20).
The extracted mRNA was used for gene expression detection with GeneChip technology and qRT-PCR was performed on the same mRNA samples using validated Assay-On-Demand primers and probes from A&B Applied Biosystems to verify the microarray results on genes of interest on the same patients included for microarray.
AH and KN mapped Microarray results on strain 8325.
There are also evidences of improved accuracy in microarray results on removal of probes with improper annotations [ 26].
IPA ranked networks depending on the reliability of the microarray results on the basis of prior published data.
Based on previous microarray results on zebra finch vocal learning and synaptic plasticity, a number of these prohormones show significant changes during learning.
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For this purpose, we compared the microarray results for samples collected on admission for the HF group versus the non-HF group.
Gene expression profiles are based on microarray results To assess the reliability of microarray data, quantitative reverse transcription PCR (qRT-PCR) was used to measure the expression level of 26 selected genes.
These results confirm the microarray results for the RA effects on periostin expression and most importantly they suggest that periostin may be playing a role in hMSC osteoblast differentiation potential since its expression can be positively regulated either by EGF+bFGF or by serum.
The present pathways analysis corroborates the original microarray results [10] on the significance of remodeling in bladder tissue after SCI, and it reveals a greater role for local and systemic inflammation than had been previously appreciated in this setting.
Microarray results, performed on HA-EC and HOC-EC, confirmed the endothelial origin of the isolated cells (Table 2).
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