Sentence examples for microarray results observed from inspiring English sources
From these results, we confirmed that the regulation of genes in bovine cells expressing Tax was similar to the microarray results observed in human cells.
In other words, SFPs between the two cultivars and their respective GeneChips may indeed be responsible, qualitatively and quantitatively, for the discrepancies between the QPCR and microarray results observed for barley but not wheat.
Indeed, although it may be noted to show the different expression patterns in several factors, such as FOS, JUN, RORA, GEM, RRAD, TNFAIP6 and IFIT3 genes in human and bovine cells, we could validate that the regulation of genes in bovine cells expressing Tax was similar to the microarray results observed in human cells in this study.
However, less agreement (Kappa = 0.2 0.6) between microarray results were observed when using different hybridization buffers, indicating this parameter as being highly critical when transferring a standard microarray assay between laboratories.
From this analysis, 100% validation of microarray results was observed (Table 1).
A significant correlation between the qRT-PCR and microarray results was observed in the combined sample of the three countries (R = 0.4 P<0.05) (Additional file 2: Figure S1C).
To further confirm the overrepresentation of antiviral genes, qRT-PCR of 15 representative genes was performed including TLRs and ISGs and the similar tendency as microarray results was observed.
Expression analysis by RQ-PCR of four selected upregulated genes correlated well with the results observed by microarray analysis (Table 3).
In order to validate the results observed by microarray analysis, fourteen randomly selected ESTs were evaluated with qRT-PCR analysis (Table 4).
These analyses confirmed the results observed in the microarray and demonstrated that primary carbon metabolism was similarly transcriptionally altered in the ΔmsgA, ΔptcD, and ΔptcE strains.
We demonstrated that the Q-PCR results of MYPOP, PSAP, IL11RA, and MUC20 were consistently correlated with the results observed in the initial microarray data (Table 3).
