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Therefore, the analysis of selected miRNAs by qPCR confirmed the microarray results, indicating the quality of the miRNA microarray.
Our results agree with previous microarray results indicating up-regulation of GADD45B as a result of adenovirus 2 infection of HeLa cells [ 9].
Further, significant positive correlations were observed between transcript expression among RT-qPCR and microarray results indicating reasonable agreement among analytical techniques.
Accordingly, qPCR analysis demonstrated that metformin upregulated 3.0-fold let-7f, which is in line with microarray results indicating a 3.8-fold miR-30b upregulation in the same experimental groups (Table 2).
Accordingly, qPCR analysis demonstrated that metformin upregulated 2.1-fold let-7f in MCS-exposed mice, which is in line with microarray results indicating a 2.7-fold let-7f upregulation in the same experimental groups (Table 2).
Similarly, qRT-PCR results were also consistent with microarray results, indicating a good correlation between the two methods, however, as commonly reported greater fold gene expression changes were observed higher (by 1.4-5.6 1.4-5.6witimesT-PCR than in gene microarray experiments.
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DNA microarray results indicate that the genes associated with oxidative stress response, nutrient starvation, and membrane functions were induced by electrochemical currents.
Microarray results indicated that genes involved in positive regulation of angiogenesis are modulated in Dbl lenses.
The microarray results indicated progressively decreasing α-actinin expression levels which were confirmed by qRT-PCR (Table 2).
For this transcript, microarray results indicated a pregnancy-related regulation, while real time RT-PCR revealed no changes in expression across the experimental conditions.
Microarray results indicate that under the conditions tested, there are minimal differences in gene expression ≥3-fold (7 genes) when comparing R. rickettsii grown in ISE6 cells to R. rickettsii grown in Vero cells.
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