Suggestions(4)
Exact(2)
Full details of the microarray results including descriptions of profiles for all isolates are given as supplementary material in Additional file 1.
The results indicated that out of 30 datapoints tested (10 genes at 3 time-points, n = 30), 28 (93.3%) were in agreement with the microarray results, including 21 (70%) with agreement in both change (down, no change, or up) and significance (P < or > 0.05) and 7 (23.3%) with agreement in change but not in significance (Fig. 3).
Similar(58)
Genes that were confirmed from the microarray results included the following (↑ indicates up-regulation, and ↓ indicates down-regulation): CG13551 (↓5d MN+glia) is involved with the defense response (Feng et al. 2009) and is also predicted to be a mitochondrial ATPase inhibitor (Tweedie et al. 2009).
The identification tags are a set of descriptors that describe the context of the expression feature, such as the experiment type (RT-PCR or microarray) and the results including prognostic category associations.
Only the 19 probes that were in disagreement between the two microarray results were included and a Yates correction (a continuity correction) was applied.
In order to assess the accuracy of microarray results, six genes including RAD51, BRIP1 (BRCA1), BRCA2, BLMand BLM and FANCD2 involved in the 'Role of BRCA1 in DNA Damage Response' pathway were selected for validation by qRT-PCR.
Previous microarray results [ 22], included in the analysis presented in this paper, can be viewed under accessions E-MEXP-206 and E-MEXP-207.
Our microarray results therefore likely include direct and indirect targets of SND2, as well as genes misregulated due to the ectopic overexpression of SND2.
The results include the analysis of three publicly available microarray databases related to cervix cancer.
Other factors that may contribute to the dissimilarity of the results include differences in probe sequence and size, microarray fabrication/chemistries, labeling/detection methods, and the vigorous mixing that is employed in our microarray experiments.
Variable results include those results found to have no significant change by microarray analysis as measured by t-test.
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