Quantitative PCR is a rapid and highly sensitive technique for accurate quantification of microarray results; however, careful consideration of experimental design, quality of primer/probe design, internal standards, and normalization procedures are pivotal, particularly when the work involves postmortem tissue.
The results of real time RT-PCR for 5 miRNAs (miR-128, miR-146a, miR-155, miR-181a, and miR-195) were consistent with our microarray results; however, the other 10 miRNAs (miR-548i, miR-708, miR-181b, miR-369-3p, miR-449a, miR-3121, miR-181a, miR-1323, miR-587, miR-181a-2 miR-181a-2 miR-181a-2 results of our micontradicteddy.
However, microarray results are influenced by various factors, and thereby should be validated by at least one traditional method [ 24].
However, the microarray results did not show any change in the expression of the proU operon.
However, the microarray results do not show any IFN mRNA targets to be affected by siQKI-tot.
However, the microarray results revealed that mRNA for AKR1B1, 1A1, 1C1, 1C2, 7A2, and 7A3 was present in the cells (data not shown).
However, the microarray results were supported by the analyses of the effects of extensive long-term ex vivo cultivation of MSC on their proliferation capacity, their morphology, surface marker profile, and differentiation ability.
However, our microarray results show no changes in BNIP3 expression levels in HT29 resistant cells (data not shown), thus suggesting that this gene does not play a role in chemoresistance in this cell line.
Regrettably, expression data is not available for genes representing branches D and E. However, the microarray results show that the remaining three loci differ in their transcriptional responses to the experimental treatments.
However, published microarray results with cultured S2 cells infected with various pathogens, including viruses, bacteria, parasites, or fungi do not demonstrate a consistent upregulation of glycerophospholipid metabolism-related genes [ 44, 47, 52].
However, consistent with microarray results, Q-PCR assays showed no significant expression changes for these genes in transgenic skin at any embryonic stages.
