As such, the microarray results give an indication of the ability of a particular clone to not only survive at a specific antibiotic concentration but also the overall fitness of that clone in the absence of any antibiotic selective pressure.
These results give further support to the application of microarrays in a routine diagnostic setting (Kohlmann et al, 2005).
Microarray results were missing or gave low ratio values for these 16 PlcR-independent genes.
Fold difference ratios from RT-qPCR results showed that twelve transcripts were congruent with the microarray results, while four genes gave no difference and four genes yielded conflicting results (Table 2).
Additional RT-PCR analyses were done using RNA samples which gave microarray results (6 probes with 8 pairwise comparisons) that were in agreement between the EC and without EC analyses (Table 3).
As noted above, the fact that microarray results are normalized to give each array the same average signal intensity will dampen the reduction in polysomal mRNA abundance in the eIF4G mutant, and the amounts of total mRNA might also decline on eIF4G depletion, which would offset the effect of decreased polysomal mRNA on the calculated TE values.
Several studies have suggested that long oligonucleotides, as long as 50 nucleotides, give satisfactory microarray results [ 5, 15].
Based on our multi-species cDNA microarray results, 1,541 transcripts in total gave positive hybridization signals in oocytes across all three species and of these, 268 transcripts in total were found to be preferentially expressed in oocytes for all three species.
Given that the microarray results indicated that one of the sources of CdTe-GSH QDs toxicity in E. coli is oxidative stress and membrane damage, the antibiotic susceptibility in bacteria previously exposed to green or red QDs was evaluated.
Trypanosomes are exceptional in that microarray results concerning steady-state mRNA levels give direct insights into post-transcriptional regulation and likely differences in mRNA half-lives.
Given the complexity of the microarray results, it is unlikely to be because of a simple difference in mRNA abundance.
