Details of genes shortlisted, on the basis of microarray results, for semi-quantitative RT-PCR.
We confirmed the microarray results for 6 transcripts (60%): INPP4B, CD74, PTPLA, NEDD9, COMMD6 and SQSTM1.
Quantitative RT-PCR was performed to corroborate microarray results for several genes.
Validation of the microarray results for known miRNAs was carried out by real-time qPCR using a TaqMan miRNA assay.
We independently validated the microarray results for two key cytokines, IL8 and MCP1, by RT-PCR analysis (Fig. 1).
We used quantitative RT-PCR (Q-RT-PCR) to verify microarray results for specific genes of interest.
Microarray results for significantly changed genes were confirmed by quantitative reverse transcriptase PCR (qRT-PCR) (Figure 1B).
This database provides experimental microarray results for this organism for four stress conditions: heat shock [28], pH [29], UV exposure [30] and tryptophan metabolism [31].
We validated the microarray results for selected miRNAs using the ALL validation cohort (N = 24, including Good = 17; Poor = 7) (Figure 3C).
Microarray results for AIED and control subjects were analyzed by comparing unstimulated PBMC, with autologous perilymph stimulated, and pneumococcal stimulated, PBMC (figure 1A).
This finding parallels our microarray results for Affymetrix probe 211372 (not shown) and our Q-RT-PCR expression of sIL1R2 in AIED and control subjects (figure 2B).
