Sentence examples for microarray results due from inspiring English sources

The expression levels of miRNAs miR-317 and miR-200b detected by qPCR were inconsistent with that of our microarray results due to unknown reasons.

Furthermore, the four genes which were excluded from the microarray results due to spot abnormalities (cpsC, ace, gelE and fsrB) were all found to be down-regulated in blood when examined by QPCR.

In order to confirm the results from the microarray experiments and to investigate the transcription of a few genes of special interest (excluded from the microarray results due to spot abnormalities), real-time quantitative RT-PCR (QPCR) was performed on 13 genes listed in Table 2.

RT-qPCR also has been traditionally used as gold standard to validate microarray results, due to its good variance coefficient in low level transcripts such as cytokines [ 18, 19].

There were no insertions or deletions found suggesting that the microarray results were not due to probe mismatches.

Quantitative differences between the QRT-PCR and microarray results were probably due to variations in efficiency of cDNA synthesis among samples, primer-dimer formations, mispriming, and lower efficiency at later cycles as amplification products compete for DNA polymerase.

In this way, the pan- Neisseria microarray eliminates false positive results due to the hybridization to non-representative probes.

However, the reliability of the microarray results is being challenged due to the existence of different technologies and non-standard methods of data analysis and interpretation.

The reliability of microarray results has been questioned due to inconsistencies in the reported data and in conclusions reached within and between different studies [ 5- 13].

However, interpreting genome-wide microarray results can be overwhelming due to the vast output of gene expression data combined with off-target transcriptional responses many times induced by a drug treatment.

Heterologous hybridisation of Pinus radiata derived probes to the Pinus microarray may produce spurious hybridisation results due to imperfect annealing that can lead to incorrect interpretations of gene expression levels; quantitative RT-PCR is therefore necessary to verify the microarray results.