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The microarray results clearly indicated involvement of ethylene-regulated genes in the phenanthrene response, but the downregulated ethylene biosynthesis transcripts ACO2 and ACS6 suggested that the PAH reduced ethylene production.
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In this respect, our inability to validate some of our discordant microarray results demonstrates that clearly other factors exist.
The microarray results (Table 1) clearly demonstrated that expression of HC-Pro in transgenic plants mimicked the effects of virus infections at the transcriptional level [ 15, 21- 26], as similar groups of genes were modulated in these plants as in Arabidopsis model plants infected by TEV [ 15] or CMV-Y [ 23].
More differentially expressed genes were found (e.g. 1,239 genes were identified as up-regulated genes in P. enysii with the P. fastigiatum reference while 394 were identified as up-regulated genes in P. enysii with the A. thaliana reference when mapping with no mismatches) and previous microarray results were more clearly confirmed (46 63% vs 28 44%).
QRT-PCR results clearly confirmed the microarray data (Table 5).
Independent confirmatory experiments by real-time RT-PCR or other techniques are clearly advisable for any microarray results obtained with a modified measurement or analysis protocol [8].
Although it is not clear why the daily profiles are slightly different when comparing results obtained using the microarray platform or qRT-PCR, the combined results clearly indicate circadian regulation in the levels of dme-miR-263a and -263b.
Our results clearly indicate that the microarray hybridization presented in this study represents a very robust method to screen whole sets of P. aeruginosa strains bearing unknown genetic variations.
Indeed, microarray analysis, together with real-time PCR validation results, clearly showed hat FXR was upregulated in pancreatic cancer tissues with LN metastasis.
The Q-PCR results clearly reflect the changes observed in the microarray analysis for all of the genes we tested (Fig. 5).
These results clearly validate the involvement of the IGF-1/Akt pathway identified in the microarray and open a new field of investigation in the pathogenesis of myasthenia in muscles.
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