Many studies have selected candidate miRNAs in EVs based on microarray results and validated them by qRT-PCR.
Pairwise comparisons were made between individual mutant microarray results and controls.
These results were concordant with the microarray results and demonstrated the absence of the long transcript in mature blood cells.
Classification based on the TaqMan® data replicated the microarray results and achieved 80% classification accuracy on the testing set.
Nine of these corroborated the microarray results and the remaining two showed a similar trend but without statistical significance.
The miRNAs chosen for validation were selected based on the significance of the microarray results and published evidence suggesting a role for the selected miRNAs in senescence.
These data validate the microarray results and substantiate the ability of Dbl oncogene to modulate genes involved in lens morphogenesis, apoptosis, angiogenesis and EMT processes.
However, a high level of concordance (r = 0.97) was observed between the microarray results and the real-time RT-PCR data (Figure 16), which confirms the reliability of the data produced in this study.
Finally, differences in the expression patterns observed in the 13 (86.7%) up-regulated and 6 (85.7%) down-regulated genes were consistent with both the microarray results and the phenotypic difference between WT and type III necrosis.
Accordingly, pairwise comparisons were made between individual Crx-/- Nrl-/- miCrx-/- Nrl-/-lts and both 129 and B6 Crx-/- Nrl-/-g the GeneCrx-/- Nrl-/-g Systemicroarrayoftwaresults andymetrix (i.e., a total of six pairwise comparisons).
In the present study we integrated morphological data with microarray results and biochemical analysis, in order to gain insight into the cellular manifestations of early events in the transformation cascade.
