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The microarray results additionally indicated regions of the chromosomes likely to contain divergent sequence, analysis of which revealed five new genes.
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Additionally, the microarray results revealed several fold up-regulation of transcripts encoding for lipoxygenases (Additional file 8: Table S5) including LOX2 and LOX2.1.
Additionally, according to our microarray results, other myosin genes were induced by iron in mouse heart (Myh7) and skeletal muscle (Myl2).
Additionally we tested whether the microarray results reflected actual expression patterns in the sample by qRT-PCR of the two major cellulase genes cel7a/cbh1 and cel6a/cbh2.
This was in line with the positive microarray results.
Microarray results were validated using real-time RT-PCR.
(A) Validation of the microarray results in both MKN-45 and SGC-7901 cells using qRT-PCR.
Validation of microarray results by semi-quantitative RT-PCR revealed temporal variation in gene expression profiles.
Fig. 4 Validation of microarray results by semi-quantitative RT-PCR.
Microarray results suggested that OsIRO3 expression may be positively regulated by IDEF1 (Table 1).
Details of genes shortlisted, on the basis of microarray results, for semi-quantitative RT-PCR.
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