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The microarray resequencing of mtDNA was 100% concordant with conventional sequencing results for 103 mtDNA variants.
Synonymous and non synonymous substitutions in mtDNA in all coding and control regions were identified in dorsolateral prefrontal cortex (DLPFC) by microarray resequencing of schizophrenia (SZ), bipolar disorder (BD), major depressive disorder (MDD), and control (C) subjects.
Zhang et al. [17] had used microarray resequencing of 1,199 genes in 10 different O157 H7 or related O157 H- strains, to estimate where the strains diverged from the Sakai/EDL933 lineage, and generated a tree with 3 branches along the major O157 H7 lineage.
The SNPs were all identically ascertained by microarray resequencing of the genome, and verified in multiple populations.
These results indicate that microarray resequencing of the mtgenome recovered from NAF is a rapid, sensitive and cost effective technique.
Microarray resequencing of human mtDNA sequences that differ by << 1% from the tiled human reference approach 100% efficiency and accuracy.
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Preparation for microarray resequencing includes pooling of amplicons at equimolar concentration, nuclease fragmentation, labeling, and fluorescent staining, according to the Affymetrix GeneChip CustomSeq resequencing Array protocol, v. 2 (2003).
The accuracy of the microarray resequencing process was defined as the ratio of the number of correctly determined bases to the total number of determined bases, by comparison with the known rpoB sequence of the tested strains.
They recommend the cautious use of microarray resequencing as an initial evaluation tool and propose to limit the use of Sanger sequencing to more problematic and discordant cases.
We investigated the efficiency and accuracy of microarray resequencing where experimental and microarray reference sequences are from different species, and the influence of the degree of sequence divergence on that performance.
In addition it was not observed in 12 mtDNA samples from control blood measured by microarray resequencing, reducing the likelihood of a microarray artifact.
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