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A Prespo/Spore ratio, measured by microarray, represents the percentage of a single deletion strain that can complete sporulation.
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In order to identify P. tricornutum genes that are differentially expressed in presence or absence of silicic acid, we developed an oligonucleotide-based microarray representing the whole set of the identified ORFs.
Labelled probes were cohybridised to a 52 000 element cDNA microarray representing the entire Unigene database.
An Agilent custom-made 60-mer oligonucleotide microarray representing the full A. gambiae transcriptome was used for the assays.
A 60-mer oligonucleotide microarray representing the entire A. gambiae transcriptome was used for these assays [ 4].
Due to the availability of the apple genome sequence [ 22], the AryANE_v1 microarray representing the fully annotated genome was recently designed [ 23].
High-throughput technologies based on the well-established DNA microarray represent the most cost-effective and convenient means to assess the gene expression profiles.
Briefly, the immunoprecipitated RNAP-associated DNA fragments were fluorescently labeled and hybridized to a high-density oligonucleotide tiling microarray representing the entire E. coli genome [ 5].
Using a genomic microarray representing the majority of the transcriptome, we profiled gene expression in stem segments that spanned primary to secondary growth.
The probes printed onto this microarray represent the coding sequences of three complete neisserial genomes (N. gonorrhoeae strain FA1090 [ 16], N. meningitidis strain MC58 [ 29], N. meningitidis strain Z2491 [ 30]) as well as the coding regions from the GGI [ 21].
Total RNA derived from the 5 stem internode development series described above was used for expression profiling with a Nimblegen microarray representing the whole annotated transcriptome at the time that the array was created.
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