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However, the methods describe duplicate technical microarray replicates, so how could it be more than 2? Please clarify.
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The data was analyzed to obtain the mean and standard error, and graphs were plotted using Prism (Graphpad Software, Inc., CA) A total of two experiments were completed with 4 chips each, so that the microarray replicates were performed in duplicate for a total of 8 chips.
The 3 experimental replicates were hybridized independently resulting in three microarray replicates per condition.
Dye-swap normalisation makes use of reverse labelling in the two microarray replicates directly [ 26].
Microarrays were replicated 6 times (3 biological replicates per treatment, each with a dye swap) and each gene was represented on the array 3 to 7 times.
Approximately half of the individual microarrays were replicated and the correlation coefficient between these technical replicates was above 0.90.
Approximately one third of the individual microarrays were replicated and the correlation coefficient between these technical replicates was 0.83 – 0.83.
Eight independently grown replicates were so tested for their MIC.
This tension replicates aspects of the so-called hermeneutic circle.
Finally, polymer microarray was successfully replicated from silicon mold.
Two biologically replicated microarray experiments were performed.
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