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The poor hybridisation of DNA from the three strains of non-proteolytic C. botulinum to the microarray reflects the wide evolutionary and phylogenetic distance between proteolytic C. botulinum and non-proteolytic C. botulinum.
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We used CGH of a spotted microarray reflecting the pan-genome of GMI1000, IPO1609, and Molk2 to further define phylogenetic relationships among a set of 51 strains selected to span the known diversity within the species complex, and to verify that the three new genomes selected were truly representative of their respective phylotypes.
These results indicate that the microarray data reflects the transcript ratios for the majority of genes.
A slope of one indicates that the microarray accurately reflects the input.
One of the greatest limitations of microarray data reflects the underlying biology: the expression of mRNAs doesn't always translate into proteins because silencing RNAs and other mechanisms can block the translation process.
There is haplotype specificity for some genes (HLA-DRB4 and HLA-DRB5) and thus we are not sure whether differential expression of HLA genes measured by microarray reflects different probe affinity for different HLA class II alleles and thus further work is needed to fully understand this association.
The gene expression profile for the resistant cells revealed with ADGE microarray reflected changes in the expression of GSTP1-1 and genes related to kinase and proliferation pathways.
The fluorescent signal of each hexamer sequence on a single microarray chip, quantitatively reflects the expansion of a certain T-cell clone.
The transcriptional or expression plasticity was estimated as the average of the squared log2 expression ratio from over 1000 microarray experiments, which reflects the capacity for a gene to change its transcriptional level under different conditions [ 34].
When using a "natural classification" strategy the only assumption being made is that the most common gene expression patterns associated with the development of diabetes are expected to be found many times, providing that a sufficiently large number of samples are included and the microarray technique accurately reflects the underlying molecular mechanisms.
RNA-seq highlighted "protein secretion by the T3SS" along with the "small molecule catabolic process", while microarray reflected "polycyclic aromatic hydrocarbon degradation" and "establishment of localization in cell", as the most significant terms of the group.
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