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All microarray reagents are from Roche Nimblegen unless otherwise stated.
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Single color (Cy3) Agilent Rat 8 × 15 K miRNA microarrays and reagents were used according to the manufacturer's protocols (Agilent Technologies, Santa Clara, CA, USA) using 100 ng of total RNA.
Single color (Cy3) Agilent Whole Rat Genome 4 × 44 K microarrays and reagents were used according to manufacturer's protocols (Agilent Technologies, Santa Clara, CA, USA) using 500 ng of total RNA.
The generation and immobilization of pure protein reagents are critical to the quality of protein microarrays.
Compared with fluorescence- and microarray-based assays, this method provides an alternative for genotyping where costly equipment or specialized reagents are not available.
All reagents are of analytical reagent grade.
Reagents: The costs of reagents are always difficult to predict.
Additional reagents are described below.
This is the case, for example, of reagents in microarray studies: "reagent" is the information to be recorded, but for different microarray studies, different types of reagents can be used.
The transition from allergen extract based to allergenic components reagents is discussed, emphasizing the chip-based microarray's strength in identifying IgE antibody cross-reactivity.
Unbound reagents were removed by washing, and the bound antibodies on the chips were visualized using the GenePix 4000B microarray scanner.
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