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Microarray protocols followed those established by the Center for Advanced Technology at the University of California, San Francisco http://cat.ucsf.edu/.ucsf.edu/
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The microarray protocol follows previously published methods [ 82, 83].
The microarray protocol follows previously published methods [ 67].
All protocols followed the MIAME recommendations [ 93] developed by the Microarray Gene expression Database Group (http://www.fged.org/).org/
Treatment protocols followed WHO international guidelines [11].
Cyanine-3 (Cy3) labeled cRNA was prepared according to Agilent's One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling protocol, followed by RNeasy Mini spin column purification (QIAGEN).
Isolation of total RNA for mosquitoes used in the microarray study followed the protocol recommended by Affymetrix Inc. (Santa Clara, CA).
This protocol follows SPIRIT 15 2013 guidelines.
Whatever the protocol, follow it.
Hybridization and washing of microarray slides followed according to the manufacturer's protocols.
For isolation of RNA from large numbers of cultured cells, standard RNA extraction and microarrays hybridization we followed standard protocols as described elsewhere [ 6, 16].
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