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The up-regulated or down-regulated changes in gene expression following GANT61 treatment and determined by cDNA microarray profiling, were confirmed by qRT-PCR (Figure 5).
Putative target genes of miR-200c identified by microarray profiling were validated as direct targets using dual luciferase reporter assays.
Animals for microarray profiling were generated from an F2 intercross between congenic males and C57BL/6J control females.
The results of microarray profiling were deposited in Gene Expression Omnibus Database under the accession number GSE64624 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi token=utmbmwkohdqvnwz&acc=GSE64624).nih.gov/geo/query/acc.cgi token=utmbmwkohdqvnwz&acc=GSE64624
Comparative genomic hybridization followed by mutational analysis, gene expression and miRNA microarray profiling were performed on 123 paired tumor and non-tumor tissue samples from patients with NSCLC.
In all cases, the cells subjected to microarray profiling were obtained from the same initial culture batch and were subsequently cultured for a total of six days before RNA was isolated and analyzed.
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Gene expression microarray profiling was performed by UCL Genomics.
The microarray profiling was performed as described in our previous work (Wang et al. 2014).
Microarray profiling was performed by Cogenics (Morrisville, NC).
MicroRNA microarray profiling was performed with RNA isolated from the Maryland cohort using the Ohio State microRNA microarray version 2.0 as previously described [9].
The microarray profiling was done to determine the global changes in mRNA expression levels in H1299 cells transfected with growth suppressive miRNAs compared to a negative control.
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