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The microarray profiling was performed as described in our previous work (Wang et al. 2014).
Microarray profiling was performed by Cogenics (Morrisville, NC).
MicroRNA microarray profiling was performed with RNA isolated from the Maryland cohort using the Ohio State microRNA microarray version 2.0 as previously described [9].
The microarray profiling was done to determine the global changes in mRNA expression levels in H1299 cells transfected with growth suppressive miRNAs compared to a negative control.
Up-regulation of stress response (MAFK, IKBZ, SOCS3) and early growth response (c-Myc, c-Jun, c-Fos, DUSP1 and 6) genes as assessed by RT-qPCR and/or microarray profiling was unchanged.
MicroRNAs microarray profiling was performed using total RNA extracted from the liver from one adult mouse (day +50) and one neonate mouse (day +1) using the LC Sciences technology (LC Sciences, Houston, TX).
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The up-regulated or down-regulated changes in gene expression following GANT61 treatment and determined by cDNA microarray profiling, were confirmed by qRT-PCR (Figure 5).
These two features, cellular origin and driving mutations, are what microarray profiling is best at detecting.
Microarray profiling is a useful strategy for examining global gene and miRNA expression [ 17].
Putative target genes of miR-200c identified by microarray profiling were validated as direct targets using dual luciferase reporter assays.
Animals for microarray profiling were generated from an F2 intercross between congenic males and C57BL/6J control females.
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