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It was not possible to directly determine how similarity to post-vaccine microarray profiles was acquired in GBM samples from non-vaccinated patients treated at other institutions.
Significant heterogeneity of VAP microarray profiles was observed secondary to patient ethnicity, age, and gender, yet 85 genes were identified with consistent changes in abundance during the seven days bracketing the diagnosis of VAP.
Second, each group of microarray profiles was normalized by using the invariant set method [ 9].
A whole genomic drug-gene interaction network based on the integrative manually curated signaling network and microarray profiles was established to explicate the potential off-target effects for torcetrapib.
That is, PCA of the microarray profiles was able to distinguish the expression changes in the strains occurred in the response to ethanol stress and in the adaptive evolution to the stress.
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As shown, the regulation of miR-1 (Figure 1A) and miR-124 (Figure 1B) in their transfection microarray profiles are both detected by our method.
Microarray profiles are stored as CEL files.
Those candidate miRNAs whose expression was consistent with the microarray profiles were selected for further analysis.
Baseline microarray profiles were generated using Agilent 60-mer oligonucleotide arrays for each cell line.
Finally, the normalized microarray profiles were integrated into a single dataset after outlier array exclusion and global median transformation.
RNA was isolated, and the microarray profiles were analyzed for differential gene expression at a 5% False Discovery Rate (FDR) [ 22].
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