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Once probe design is complete, and the whole microarray procedure is optimized as a standard procedure, pathogen screening results can be interpreted in less than 28 hours.
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Microarray Procedure was compliant with MIAME.
The microarray procedure was carried out as described previously.
All of the microarray procedure was MIAME (Minimum Information About a Microarray Experiment) compliant.
The microarray procedure was conducted at the University of Illinois Urbana-Champaign at the Keck Center for Biotechnology.
Full details of the microarray procedure are available in the Methods section.
A more limited series of confirmatory relative gene expression experiments, focused specifically on those genes identified as being significantly up- or downregulated using the microarray procedure, were also performed.
A calibration curve, consisting of several dilutions of cDNA of the reference used for the microarray procedure, was included for each gene to allow calculation of the relative starting quantities.
Standardization of every step in the DNA-microarray procedure is crucial to correctly and efficiently perform DNA-microarray experiments, and to obtain reproducible data [ 10- 13].
All microarray procedures were done at our microarray facilities.
The same basic microarray procedures were used in both species (see Methods).
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