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The complete microarray procedure has been fully described before (de Jong et al. 2006).
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The detection sensitivity in the microarray experiment procedure has been reported as a number of transcripts per cell, when 1 5 μg of target was used [ 13].
Such a procedure has also been reported as necessary for pooled analyses on microarrays [ 15].
The procedure has certain risks.
This procedure has many advantages.
This procedure has 5% superfat.
Although conventional microarray analytical procedures have proved adequate in handling M-CGH data, data interpretation using these methods is based on a continuous character model in which gene divergence and gene absence form a spectrum of decreasing gene conservation levels.
All data concerning the shotgun genomic DNA microarrays used and the hybridization procedure have been deposited in the GEO repository complying MIAME (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi acc=GSE11269).nih.gov/geo/query/acc.cgi acc=GSE11269
7, 8 The details of the microarray platform used and the hybridization procedure have been reported previously.
A first analysis of the data indicated that the hybridization procedure had failed for two of the eight microarrays.
The entire microarray procedure and its analysis have been validated and reported in detail [ 6].
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