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The same RNA samples isolated from peripheral blood monocyte precursors and immature DC (iDC) were used for GeneChip microarray probing and SSH cDNA library construction.
The same RNA samples isolated from immature DC (iDC) and RNA samples isolated from monocytes were used for GeneChip microarray probing and SSH library construction.
Similar(58)
Similarity between microarray probes and probed DNA was estimated via probes of alleles of marker genes gyrB, mutS, and recA spotted on the microarray.
The partial GABAB1k clones contain the microarray probe and GABAB1k unique region, 3' intron 4 of GABAB1a.
Mismatches between a microarray probe and its target sequence affect hybridization that cause erroneous probe signal estimates [28].
This may be explained by the inability of our microarray probes and PCR primers to discriminate between IRE-positive and IRE negative transcripts.
The WB IDs are linked to the physical coordinates in the genomes, as are microarray probes and RNAi clones, so that these automatically follow evolutions in gene structure.
Fragments of approximately 1000 bp encompassing the target sites for microarray probes and qRT-PCR primers in the genes TP03_0869, TP03_0883 and TP04_0921 of the cell lines 951-A3, 951T-F31, 951T-F31, 951-I8 and 951T-S80.9 were sequenced.
The microarray probes and the assay "ex7-8" detect a mix of all splicing forms of TCF7L2, while the assays ex12-133", "ex13-13andand "ex3a-4" distinctisplicingplicing forms that showed difference in expression between the two types of adipose tissue.
NM wrote the software used for probe design, designed microarray probes and performed microarray hybridizations.
PCR probes were produced as previously described for microarray probes and analyzed on agarose gel.
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