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The microarray probes used in the present study were based on the EGDe strain (Accession No. A-BUGS-19; http://bugs.sgul.ac.uk/A-BUGS-19).
To test if any of our results may be affected by the presence of SNPs within the microarray probes used to measure expression levels, we compiled a list of 1,428 probes overlapping known SNPs (3.6% of all probes).
(B) Microarray probes used for qRT-PCR validation and the annotation.
The microarray probes used in the studies in Oncomine do not distinguish between neonatal and adult SCN5A splice variants.
This two to seven-fold decrease in the expression level of both genes from south Mozambique to Malawi and to Zambia was observed across the three distinct microarray probes used for each gene.
The crux of the problem appears to be the large number of proliferation-related genes in the breast cancer transcriptome itself, given that the authors found that 58% of the microarray probes used for the analysis of the NKI-295 dataset were correlated with meta-PCNA [ 11].
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The differential expression of transcripts (treated vs. untreated) was determined from unfiltered microarray probes using the Benjamini-Hochberg method with adjusted p < 0.01 [28].
BLAST alignment of microarray probes (using criteria as described above) was used to identify which of the 4544 probes are hybridizing to genes shared among sequenced S. aureus genomes (COL, N315, MW2, Mu50, MRSA252 and MSSA476).
We applied the CAMERA BLAST search tool with subsequent selection of resulting hits for potential cross-hybridization with microarray probes, using the following criteria: (i) an alignment length of at least 30 nucleotides with E value of less than 0.05%, and (ii) at least 90% identity score for the query-match alignment.
Gene ontology (GO) terms were allocated to each gene represented by the sequenced microarray probes using Blast2GO (http://www.blast2go.com/).
P-values were adjusted for multiple testing across all the microarray probes using the Benjamini-Hochberg method.
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