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Our RUD values were based on microarray probes targeting cUTR and aUTRs that are defined by proximal and distal poly(A) sites.
Our method to analyze 3'UTR regulation was based on comparison of microarray probes targeting cUTRs with those targeting aUTRs with respect to intensity changes between samples in a sample set (Figures 1 and S1).
Microarray probes targeting Pseudomonas clusters, some Gammaproteobacteria, and some Betaproteobacteria contributed significantly to the hybridization patterns observed for the Kool Filter Kings and Lucky Strike Original Red cigarettes, which distinguished them from the Marlboro Red and Camel samples.
This paper shows that microarray probes targeting transcription from the complementary strand of known genes can identify novel NATs, an approach that has not been possible solely on the basis of publicly available cDNA data.
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In the present study, our microarray probes targeted 407 discrete transcribed regions of four human HOX loci.
We hypothesize that RT-PCR expression values show behavior of only one of the alternative transcripts (green line), while microarray probes target the alternative transcript (probe b, red line) and both transcripts (blue line).
Northern blots and sequencing revealed that the microarray probe targeting the 3' end of the mRNA molecule detected alternative splicing of the last exon and not differential expression.
A functional gene array is a DNA microarray containing probes targeting sequences unique to genes within families of interest.
Expression microarrays use probes targeting specific genes based on nucleotide sequence complementarity to quantitatively measure mRNA levels for tens of thousands of genes.
The main assumption made in design of a microarray is that probes targeting a single transcript are placed randomly on its surface.
The availability of whole genome sequence of B. rapa[ 27] facilitated the design of a 60-mer oligonucleotide microarray platform (62,654 probes targeting 42,162 Brassica genes) based on predicted gene models from the genome sequence.
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