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Importantly, BLAST analysis of the microarray probes suggested that they measured their respective FeSOD transcripts uniquely.
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This was done in a careful manner by taking detection of differential expression in one progression state as evidence for the other and included a gene in the extended set if the detected microarray probe set suggested more than 2-fold expression change.
Preliminary examination of hybridization probe specificity suggested that several microarray probes had the potential to hybridize to multiple targets.
The solution phase-type hybridization behavior on the microarray surface suggested that probes on this surface should exhibit typical hybridization kinetics, where the reaction rate is dependent on the second order rate constant, the target concentration, and the probe concentration [ 19, 20].
The set of sequences selected above provides a valuable resource for designing microarray probes, and the current widespread use of microarrays for gene expression profiling of corals [ 32, 33] suggests that this resource will be immediately useful.
Microarray probes were matched to gene identifiers using the CDF array annotation (version 18) provided by the University of Michigan microarray© lab56.
Fig. 3 Transcripts identified in RAP annotations and 44 K microarray probes.
In both root and shoot samples, about 95% of transcripts were supported by the microarray probes.
Prepared microRNAs were hybridized to microarray probes.
We named these microarray probes as WABMs.
CS designed and annotated microarray probes.
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