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Microarray probes required perfect homology across all of their 25 bases to produce a positive match.
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The SNP platform used to generate our data measured 500,568 SNPs, and the gene expression microarray platform measured gene expression levels across 54,675 probes, requiring statistical evaluation of more than 13 × 109 comparisons.
Heterologous hybridisation requires microarray probes that are capable of tolerating a certain degree of mismatch with the target transcripts.
As genes belonging to these groups tend to have different paralogs, better understanding of the results will require mapping contigs and microarray probes to the Spalax genome when it becomes available.
If we stipulate that state of the art microarray probe design procedures usually require that all probes have similar GC content, resulting in very similar hybridization profiles[27], on a well-designed array global a, b and d may adequately represent the individual probe properties.
The nomenclature system requires unique identifiers as well as consistent annotation for RNA control sequences and the related PCR reagents and microarray probes across various platforms.
Fig. 3 Transcripts identified in RAP annotations and 44 K microarray probes.
In both root and shoot samples, about 95% of transcripts were supported by the microarray probes.
The target gene regions of microarray probes were retrieved from the annotation file for human U133 Plus 2.0 (http://www.affymetrix.com).
Prepared microRNAs were hybridized to microarray probes.
We named these microarray probes as WABMs.
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