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Each gene is represented by 3 microarray probes placed at exon-exon junctions or in exons.
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Microarray data were derived from custom microarray patterns, with probes placed in every exon and exon-exon junction, and a random-priming amplification protocol (Materials and Methods).
Jetset: Selecting the optimal microarray probe set to represent a gene.
After fluorescent labeling, the RNA was hybridized to a microarray probe set consisting of 60 sequences targeting different regions of the P. stutzeri 16S rRNA gene.
The target gene regions of microarray probes were retrieved from the annotation file for human U133 Plus 2.0 (http://www.affymetrix.com).
a Chromosomal position as middle of two microarray probes.
bChromosomal position as middle of two microarray probes.
The microarray slide was placed probe side towards the target.
Microarray probes were matched to gene identifiers using the CDF array annotation (version 18) provided by the University of Michigan microarray© lab56.
Fig. 3 Transcripts identified in RAP annotations and 44 K microarray probes.
In both root and shoot samples, about 95% of transcripts were supported by the microarray probes.
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