Suggestions(1)
Exact(1)
A total of 23,195 microarray probes passed the differential expression significance threshold (see Methods).
Similar(59)
For the 60-mer probes, 33,740 (54.5%) sense probes and 28,133 (45.5%) antisense probes passed the process.
Only probes with IQR/median>1/8 were selected for analysis, and 22 820/47 231 probes passed filtering.
Replicate probes were mean-summarized and all probes were required to pass Agilent QC flags for all replicates of at least one infected time point (27,912 probes passed).
In normal tissue 25735 probes passed and in tumor vs normal tissue 27919 probes passed filter on flags for further analysis with t-test (p < 0.05).
For the HumanMethylation27 BeadChip arrays, 25,922 (94%) methylation probes passed quality control; for the HumanMethylation450, 441,716 (91%) methylation probes passed quality control.
Conserved read probes passing the statistical tests for microarray hybridization were annotated using Blast2GO tool [ 42].
Microarray CGH analysis software usually requires 3 continuous probes passing a log2 signal ratio threshold in order to call an aberration.
An additional 8,942 probes with consensus detection calls of AA across all developmental stages were also removed, leaving 22,507 probes passing filters (57 % of the microarray probe sets).
RNA was purified from subcutaneous adipose tissue and the microarray data was normalized using the Microarray Quality Control Pipeline, as previously described [ 10], resulting in 14,942 probes passing quality control.
To prevent potentially confounding factors influencing expression values, gene expression values were corrected for age, sex, and twin relatedness using the Microarray Quality Control Pipeline as described previously [ 10], resulting in 16,048 probes passing quality control.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com