Your English writing platform
Discover LudwigSuggestions(1)
Exact(1)
Methylation levels are reflected in the log2 (HpaIIresistant(r)/McrBCresistant(r)) value for each microarray probe (see Materials and Methods for details).
Similar(59)
On the advanced search page, an additional form is provided that can be used to retrieve information on microarray probes, Figure 4D (see Additional file 4).
Several novel algorithms were therefore developed to assign new annotations for microarray probe-sets (see Materials and Methods): of the 1678 microarray probe-sets for which we identified new annotations, 93% were associated with genes already represented in our highly significant gene lists.
However, the results of our reciprocal BLAST search (see below) indicated that SCO6832 is present in S. lividans TK24 and diverges only from the S. coelicolor gene sequence at the 5' and 3' termini in a pattern consistent with that of the microarray probe-binding (see Additional File 6).
We compared fold changes of the average of values for the qRT-PCR on the candidate exons with microarray probe set inclusion levels (see examples in Figure 6, compare blue lines with red bar graphs, respectively).
The microarray gave positive signals for 46 probes (see Figure S1 in Additional file 1), corresponding to 40 different pre-miRNAs.
P-values (P) for over- (OR > 1) or under-representation (OR < 1) of QTL candidate genes in list of selected genes were calculated using the genome and microarray probes as reference (see methods).
Because the microarray had 2-3 fold genome coverage, most genes were redundantly represented by several overlapping probes (see also Figs. S5 to S7).
We have updated our microarray dataset to reflect the new gene numbers where probes originally designed to intergenic regions are now acknowledged to target a newly annotated gene (see Additional file 4 for microarray probe gene assignment update and Additional files 5 and 6 for details).
There are several possible explanations for disagreement between microarray and QPCR results (e.g. differences between location of QPCR amplicon and microarray probe or possible misassembly of contigs, for more details see the discussion of Booman et al. [ 21]).
We saw progressively increasing biological reproducibility of gene expression measurements when we initially used these quality scores to identify the best microarray probe for each gene, and subsequently excluded genes from the analysis with no reliable probes.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com