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Mismatches between a microarray probe and its target sequence affect hybridization that cause erroneous probe signal estimates [28].
The partial GABAB1k clones contain the microarray probe and GABAB1k unique region, 3' intron 4 of GABAB1a.
The ERCC Informatics subgroup will determine the "correct" sequences to be used for microarray probe and QRT-PCR primer design.
RNA-seq does not depend on the hybridization chemistry of a microarray probe and therefore can detect expression variation over a large dynamic range [ 3].
The probe sequences were designed within the sequence of the corresponding microarray probe, and anneal to the cDNA sequence of the respective gene.
To remove the CpG density bias from smoothed SLRs, we determined a coupling factor for each microarray probe and calculated relative methylation scores (RMSs) that are free from known immunoprecipitation biases due to variable CpG densities.
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Similarity between microarray probes and probed DNA was estimated via probes of alleles of marker genes gyrB, mutS, and recA spotted on the microarray.
This may be explained by the inability of our microarray probes and PCR primers to discriminate between IRE-positive and IRE negative transcripts.
The WB IDs are linked to the physical coordinates in the genomes, as are microarray probes and RNAi clones, so that these automatically follow evolutions in gene structure.
Fragments of approximately 1000 bp encompassing the target sites for microarray probes and qRT-PCR primers in the genes TP03_0869, TP03_0883 and TP04_0921 of the cell lines 951-A3, 951T-F31, 951T-F31, 951-I8 and 951T-S80.9 were sequenced.
The microarray probes and the assay "ex7-8" detect a mix of all splicing forms of TCF7L2, while the assays ex12-133", "ex13-13andand "ex3a-4" distinctisplicingplicing forms that showed difference in expression between the two types of adipose tissue.
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