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Protein microarray printing and quality control protocols have been previously described.
The main drawback of the microarray technology is the necessary large initial investment for sophisticated and expensive instruments needed for microarray printing and laser scanning.
Further, it can be used for quality control of microarray printing and PCR product quality, detection of hybridization anomalies, and simplification of spot finding and segmentation tasks.
We are greatly indebted to Anne Haegert and Dr. Stéphane Le Bihan at The Jack Bell Prostate Centre, Vancouver, for microarray printing and quality control.
We show that vRNA can also be used for quality control of microarray printing and PCR product quality, detection of hybridization anomalies, and simplification of spot finding and segmentation tasks.
Results of this study show that a reference sample (vRNA) derived from the parental EST clone vector of all clones printed on an array provides a useful universal reference that can be used for quality control of microarray printing and PCR product quality, detection of hybridization anomalies, and simplification of spot finding and segmentation tasks.
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Details of the preparation of the microarray prints and image acquisition have been described previously[ 5].
Furthermore, the arrays are simple to produce and applicable to various commercially available polystyrene cell culture vessel formats and moreover scalable, limited only by the microarray printing capacity and throughput of the image analysis systems.
Also thanks to Prof Andrew Cossins (University of Liverpool) for the use of their microarray printing facilities and Dr Margaret Hughes for printing.
They were immobilized onto the CodeLink activated microarray slide (Amersham Biosciences Corp., NJ) with 10 technical replications of each concentration according to the manufacturer's instruction using MicroGridII microarray printing system and BioRobotics MicroSpot 2500 pins (Genomic Solutions, MI) at 40% relative humidity.
Detailed protocols for glass slide coating, cDNA clone preparation and verification, microarray printing, post-printing slide processing, RNA extraction, RNA amplification, labeling and hybridization have been published by our laboratory (Selaru et al. 2002b; Xu et al. 2002; Zou et al. 2002).
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