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We thank S. Hand for use of the pizeo-electric microarray printer and for critical but insightful thoughts and reading of the manuscript and G. Churchill, A. Whitehead, A. Clark and M. Q. Martindale for discussions and critical reading of the manuscript.
Oligonucleotides were spotted on Corning UltraGAPS slides in 3× SSC buffer, using a GeneMachines OmniGrid (Genomic Solutions, MI, USA) microarray printer, and a Qiagen BioRobot 3000 liquid handler (LabX, ON, Canada) to manage oligonucleotide libraries (Operon Biotechnologies, AL, USA).
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In this study, we used the robotic application of the enzyme using the microarray printer with 100 μm spot-to-spot spatial resolution and the mass spectral images were acquired with spatial resolution of 100 μm.
Probes (neoglyconjugates, glycoproteins and lectins) were printed on Nexterion® Slide H (Schott, Mainz, Germany) using a SciFlexArrayer S3 piezoelectric microarray printer (Scienion, Berlin, Germany) under constant 62% (+/−2%) humidity at 20 °C essentially as previously described57,58,59.
Protein microarrays were printed onto nitrocellulose-coated glass FAST slides (Whatman) using an Omni Grid 100 microarray printer (Genomic Solutions).
The microarrays were printed with a Genetix QArray Mini microarray printer using 150 µm solid aQu pins onto Genetix Amine slides.
Microarray printing was performed with a Genetix Qarray2 (Genetix Ltd) microarray printer using 16 solid tip pins with 200 μm tip (PointTechnologies).
Microarrays were printed onto nitrocellulose coated glass slides (FAST from Schleicher & Schuell Bioscience) using an Omni Grid 100 microarray printer (Gene Machines).
All cDNAs were printed as single spots on EZ Rays aminosilane slides (Matrix/Apogent Discoveries) with the Biorobotics Microgrid II microarray printer (Genomic Solutions).
N-linked glycans are then selectively released from glycoproteins of tissue section by applying PNGase F enzyme using a microarray printer.
A set of 10,642 cDNA amplicons was produced and printed in duplicate side-by-side columns on Corning UltraGAPS slides with a Chipwriter Pro microarray printer (Bio-Rad) at the Biotechnology Research Institute (BRI), National Research Council Canada (NRC).
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