Microarray prehybridization was performed in 5 × SSC (SSC: 150 mM NaCl, 15 mM Na-citrate, pH 7.0), 0.1% SDS, 1%BSA at 42°C for 45 min (Fluka, Sigma-Aldrich, Buchs SG, Switzerland).
Synthesis of the two differentially labeled cDNA pools (wild-type MR-1 and the Δ so2426 mutant) to be compared, microarray prehybridization and hybridization, and post-hybridization washings were performed as described previously [ 36].
Microarray slides were pre-warmed in microarray prehybridization buffer (50°C for 30 min), and transferred into hybridization chambers (Corning, Lowell, MA, USA) and lifter cover slips (Erie Scientific, Portsmouth, NH, USA) were laid over the probe areas.
Prehybridization of microarrays was performed in a hybridization buffer (5× Denhardt's, 5× SSC, 0.5% SDS) at 65°C for 4 hours.
Microarray analysis was performed by Microarray Facility Tübingen, Germany.
Microarray was performed as described previously [20].
Prehybridization was performed by incubating the arrays with prehybridization solution (6× SSPE, 0.05% Tween-20, 20 mM EDTA, 5× Denhardt's solution, 100 ng/μl Salmon Sperm DNA, 0.05% SDS) for 30 min at 45°C.
Microarray analysis was performed using a 21.8K spruce cDNA microarray.
Microarray experiments were performed using Agilent whole-genome Arabidopsis microarrays.
Sample preparation and microarray hybridisation was performed as described previously,[15] apart from the different postprocessing and prehybridization described above.
