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There are two major microarray platforms that have been widely used: cDNA microarrays and oligonucleotide microarrays.
The bias appears to be common to most microarray platforms that use amplification and mean intensities of one to nine percent of the probes increase aberrantly to levels up to a hundred fold the average level of unaffected probes.
Several reports and reviews have pointed out the differences that exist between different microarray platforms that are in use for miRNA profiling, RT-PCR techniques, and different miRNA isolation kits in today's market from different vendors that can interfere with our ability in comparing our results with other reports[67].
Microarray platforms that utilize oligonucleotide DNA probes corresponding to predicted open reading frames (ORFs) have provided a reproducible approach to analyze changes in mRNA levels, allowing meaningful analyses of patterns of gene expression in a variety of cellular systems and model organisms [37].
There are two commercially available high-density microarray platforms that use short oligonucleotides for expression profiling.
This study compared the gene coverage of mouse whole genome microarray platforms that are currently available to investigators.
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To develop a microarray platform that allows simultaneous assessment of aneuploidy and quantification of mitochondrial DNA (mtDNA) in human polar bodies and embryos.
The NKI295 samples sets were analyzed on a different microarray platform that provided a reduced set of overlapping genes for the survival analysis.
However, presently there are only a few papers on activation-dependent genome-wide alternative splicing in human T lymphocytes, using the Agilent 44K microarray platform that allows monitoring of >5000 cassette-type AS events in human cells [28], as well as Exon 1.0 ST array to study the genome-wide effect of silencing a splicing factor during T cell activation [48].
We measured expression changes during diploid progenitor species divergence, allopolyploid formation and parallel domestication of diploid and allopolyploid species, using a microarray platform that interrogates 42,429 unigenes.
Mootha et al. [ 1] studied a group of older diabetes subjects (approximately 66 years) using a microarray platform that provides greater coverage of the transcriptome (approximately 20,000 sequences).
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