Sentence examples for microarray platforms only from inspiring English sources
For all microarray platforms, only genes whose probes had 100% identity to cDNA sequences were used.
In order to make a more direct comparison between the two microarray platforms, only red (Cy5) channel signals were used for illustrating the reproducibility of signal intensity for Agilent arrays in these plots.
Since the microarray platform only generates relative expression values, the correlation between the RNA-seq data and the microarray data was calculated using the log2 value of the ratio between A431 and U251, which in the RNA-seq case yields one value per Ensembl-gene.
To this end it should also be noted that the MammaPrint microarray platform only includes the prognostic signature genes and a set of housekeeping genes used for normalization purposes.
So we do believe our work, by involving not only 4 different microarray platforms, but more importantly also a deep sequencing technology, does yield new knowledge.
Hence, it is important to evaluate microarray platforms not only based on their production characteristics but also using a variety of analytical and statistical methods.
Moreover, these studies were based on the old microarray platforms with only approximately 7,000 genes represented on the chip, less than half of the present annotations.
Consequently, data produced so far using different microarray platforms, can only be considered by focusing on the highly differentially expressed genes.
Both mass spectrometry devices for proteomics and metabolomics profiling as well as gene and protein microarray platforms can only provide a limited reproducibility (Albrethsen, 2007; Chen et al., 2007).
The most significant disadvantages of microarrays include the high cost of a single experiment, the large number of probe designs based on sequences of low-specificity, as well as the lack of control over the pool of analyzed transcripts since most of the commonly used microarray platforms utilize only one set of probes designed by the manufacturer.
This study used a different microarray platform with only about half of the genome represented on the array.
