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We identified orthologous relationship for 3.861 genes across the microarray platforms of these two studies based on the best reciprocal BLASTN hit between cDNA sequences and identical mapping of cDNA sequences to gene IDs (see Methods).
Currently, various tiling microarray platforms of common model organisms are well supported by commercial vendors, and many bioinformatics tools have been developed for ChIP-chip analysis [ 11- 14].
BioLink MatchMaker uses NCBI's UniGene Cluster IDs to identify probes which represent equivalent genes on two different microarray platforms of the same species stored within GPX-MEA.
Identification of corresponding probesets in the microarray platforms of A. thaliana, rice, poplar, grapevine and citrus were performed using the BLAST software NCBI-BLAST-2.2.29+ NCBI-BLAST-2.2.29+ NCBI-BLAST-2.2.29+16s0100g00290) and Class II (VIT_16s0100g00290) SDH sequences with default settings.
For establishing the feature sets used in the random forest classification approach we considered only genes present on both microarray platforms of the two different studies (Affymetrix Human Genome U219 Array Plates in the case of the data of Kulis et al. and Affymetrix Human Genome U133A Arrays in the study of Fabris et al. n = 12,307).
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Probes and hybridizations were performed by the Microarray platform of the IGBMC and Génopole Alsace-Lorraine (http://www-microarrays.u-strasbg.fr).fr
Affymetrix GeneChip® Mouse Genome 430 2.0 arrays were screened with six generated probes via the microarray platform of McGill University and Génome Québec Innovation Center as previously described [14] (data are accessible through GEO series accession number GSE11759).
Microarray experiments were performed on the microarray platform of Institute of Research in Biotherapy at the Montpellier University Hospital.
Microarray experiments were performed in DNA microarray platform of our Institute of Research in Biotherapy at the Montpellier University Hospital.
These were screened on a microarray platform of 60mer MUC1 glycopeptides and recombinant MUC1 containing 16 tandem repeats.
Khan et al [ 4] dataset has been obtained on custom cDNA microarray platform of 6,567 probes [ 44] and consists of 26 RMS samples from cell lines and biopsies.
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