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External RNA controls (ERCs), which can be either added to the total RNA level (tERCs) or introduced right before hybridization (cERCs), are designed and recommended by commercial microarray platforms for assessment of performance of a microarray experiment.
There is great current interest in developing microarray platforms for measuring mRNA abundance at both gene level and exon level.
In the present study, the proposed method for determining gene signature is straightforward and independent of different microarray platforms (for example various uncommercial cDNA chips and Affymetrix chips).
Over the past decade, more than 3,000 scientific publications have reported results using the Affymetrix GeneChip arrays (Affymetrix, Inc., Santa Barbara, CA) alone, one of the most frequently used microarray platforms for expression profiling [1].
The newly released genotypic data from the 1000 Genomes Project provides an opportunity to systematically evaluate the potential influence of common genetic variants on these microarray platforms for their use in human samples.
To compare expression across species, genes were required to have orthologs in the human, rhesus macaque and mouse genome databases, and to have probesets in the microarray platforms for each species.
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In this study, we test the suitability of a high-density oligonucleotide microarray platform for direct, quantitative detection of GMOs found in the Turkish feed market.
We report here a new in situ synthesized oligonucleotide-based microarray platform for Mycobacterium tuberculosis that has been updated for the latest genome information and incorporates hitherto unannotated genes with described biological functions.
Unlike the previous experiment, the microarray platform for this dataset is the RAE230A, which consisted of 15923 probesets.
The Affymetrix GeneChip is a commonly used microarray platform for genome-wide expression studies.
Ericsson et al. [ 17] used a dual-tag microarray platform for high-performance nucleic acid analysis.
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