We investigated miRNA expression profiles in cervical cancer using a microarray platform containing probes for mature miRNAs.
A genome-wide microarray platform containing the predicted coding sequences (putative genes) for T. thermophila is described, validated and used to study gene expression during the three major stages of the organism's life cycle: growth, starvation and conjugation.
The microarray platform contained 1,262,421 probes that could each be unambiguously mapped to a single location (i.e., uniquely mapped) in the maize genome and that we therefore concluded are non-repetitive [22].
To assess the possibility that the small-sized RNAs contained microRNAs, we utilized a spotted microRNA microarray platform containing DNA oligonucleotides representing 196 human microRNAs [12] to interrogate the microRNA contents of mature erythrocytes and K562, an erythroleukemia cell line which expresses many genes typical of an earlier stage of erythroid differentiation.
Furthermore, our microarray platform contains several recently discovered miRNAs, making it supplementary to other platforms.
The HT_HG-U133A microarray platform contains 22,268 Affymetrix probe sets representing 13,262 genes.
This microarray platform contains 700,000 unique 25-mer oligonucleotide features (spots) representing 27,342 Entrez Gene IDs.
The microarray platform containing 8088 70-mer oligo probes was described previously in [ 15].
Microarray experiments employed the INDAC dual channel DNA microarray platform, containing 18,240 oligonucleotide probes (Gene Expression Omnibus (GEO) accession: GPL5016), prepared in the FlyChip microarray facility, University of Cambridge.
Expressed and differentially regulated miRNAs were initially identified using a miRNA microarray platform containing probes against 2,367 mature miRNAs from human, mouse, and rat.
